| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Robinson, Maya S (12)
|
MDFAST |
33.72 |
36.43 |
2.71 |
| 2 |
Price, Delaney G (12)
|
MDFAST |
36.41 |
38.90 |
2.49 |
| 3 |
Gonzalez, Olivia G (12)
|
MDACA |
38.77 |
39.44 |
0.67 |
| 4 |
Longo, Mia C (12)
|
MDACA |
41.47 |
39.46 |
-2.01 |
| 5 |
Kacher, Paige H (11)
|
MDACA |
47.07 |
43.97 |
-3.10 |
| 6 |
Marra, Emmeline L (12)
|
MDFAST |
42.93 |
44.05 |
1.12 |
| 7 |
Carter, Elena L (11)
|
MDBAAC |
46.81 |
44.61 |
-2.20 |
| 8 |
Mann, Grace E (12)
|
MDACA |
43.91 |
44.80 |
0.89 |
| 9 |
Marra, Adalynne M (11)
|
MDFAST |
48.87 |
46.26 |
-2.61 |
| 10 |
Mitchell, Ashley M (12)
|
MDBAAC |
49.48 |
47.08 |
-2.40 |
| 11 |
Steinkamp, Anais K (11)
|
MDFAST |
|
49.27 |
- |
|
Clement, Charlotte K (11)
|
MDACA |
55.08 |
NS |
- |
|
Lombardo, Isabella G (12)
|
MDACA |
|
NS |
- |
|
South, Amelia G (11)
|
MDACA |
46.49 |
NS |
- |